This toolkit is designed to assist medical and mental health professionals, health administrators, government officials, shelter staff, and other care providers in assessing and improving health care services available to trafficked children and adults, either on-site at their own organization, or at one or more local facilities (‘referral network’). The kit contains four sections: (1) an overview of human trafficking, (2) a service-assessment tool for determining strengths and challenges in a given facility’s medical and/or mental health care delivery, (3) guidelines for developing or improving medical and mental health services for trafficked persons, and (4) a template for organizing the names of key local and national partners and their contact information. This list will help ensure comprehensive care for trafficked persons.